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比色法GAPDH分析试剂盒
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a tetrameric enzyme that catalyzes glycolysis and
thus serves to break down glucose for energy and carbon molecules. Since it is ubiquitously expressed at
relatively constant levels, GAPDH gene is widely used as an internal control for RNA and protein analysis. The
ScienCellTM Colorimetric GAPDH Assay provides a non-radioactive method to measure the enzyme activity of
GAPDH in cultured cells, based on the oxidization of β-NADH to β-NAD in the presence of 3-phosphoglyceric
acid (3-PGA), adenosine 5’-triphosphate (ATP) and GAPDH. The GAPDH activity is determined by assaying
the rate of NADH oxidation, which is proportional to the reduction in absorbance at 340 nm over time
(ΔA340nm/min).
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a tetrameric enzyme that catalyzes glycolysis and 
thus serves to break down glucose for energy and carbon molecules. Since it is ubiquitously expressed at 
relatively constant levels, GAPDH gene is widely used as an internal control for RNA and protein analysis. The 
ScienCellTM Colorimetric GAPDH Assay provides a non-radioactive method to measure the enzyme activity of 
GAPDH in cultured cells, based on the oxidization of β-NADH to β-NAD in the presence of 3-phosphoglyceric 
acid (3-PGA), adenosine 5’-triphosphate (ATP) and GAPDH. The GAPDH activity is determined by assaying 
the rate of NADH oxidation, which is proportional to the reduction in absorbance at 340 nm over time 
(ΔA340nm/min). 
 
Kit Components 
 
Cat. No. # of vials Name Quantity Storage 
8148a 1 Cell Lysis Buffer 10 ml 4oC 
8148b 1 GAPDH Assay Buffer 12.5 ml 4oC 
8148c 1 100 mM 3-PGA 1 ml -20oC 
8148d 1 100 mM L-Cysteine 0.5 ml -20oC 
8148e 1 7 mM β-NADH 0.25 ml -20oC 
8148f 1 34 mM ATP 0.5 ml -20oC 
8148g 1 400 units/ml 3-PGK 0.125 ml -20oC 
8148h 1 GAPDH standard (200 units/ml) 20 ul -20oC 
 
Product Use 
 
This assay kit is used to evaluate GAPDH activity in vitro. It is for research use only. Not 
for use in animals, humans, or diagnostic procedures. 
 

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